Recombinant Human ICOS ligand (ICOSLG), partial (Active)
Recombinant Human ICOS ligand (ICOSLG), partial (Active)
Product Details
| Purity | ≥ 95% as determined by SDS-PAGE. |
|---|---|
| Endotoxin | Less than 1.0 EU/ug as determined by LAL method. |
| Target Names | ICOSLG |
| UniProt No. | O75144 |
| Alternative Names | ICOSLG; B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD antigen CD275 Molecular Characterization |
| Source / Host | Mammalian cell |
| Tag | C-terminal 6xHis-tagged |
| Protein Length | Partial |
| Molecular Weight | 28.9 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
| Reconstitution | We recommend briefly centrifuging the vial prior to opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile deionized water to a recommended concentration of 0.1–1.0 mg/mL. If used immediately after reconstitution, glycerol is not required. If not for immediate use, after the protein is completely dissolved, add glycerol to a final concentration of 5%–50% (default: 50%), aliquot as needed, and store at -20℃/-80℃ for long-term storage. If dilution is needed during subsequent use, dilute with the same buffer as the protein, but without stabilizers (e.g., glycerol, trehalose, etc). Troubleshooting and FAQs Protein FAQs |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Lead Time | 3-7 business days |
Description
ICOSLG (B7-H2) plays a critical role in T cell costimulation and is implicated in tumor immune evasion, making it a key target for immuno-oncology research. This recombinant human ICOSLG fragment (aa 19–258) demonstrates robust binding to its cognate receptor ICOS with an EC50 of 29.04–43.59 ng/ml in functional ELISA, supporting use as a coating antigen for antibody screening, an ELISA positive control, or a ligand in T cell activation and proliferation assays using primary PBMCs or purified T cell subsets. Produced in mammalian cells with a C-terminal 6×His tag, the protein retains… Read more