Recombinant Bovine Fibroblast growth factor 2 (FGF2) (Active)
Recombinant Bovine Fibroblast growth factor 2 (FGF2) (Active)
Product Details
| Purity | ≥ 95% as determined by SDS-PAGE. |
|---|---|
| Endotoxin | ≤200 EU/mg by the LAL method |
| Target Names | bFGF/FGF-2 |
| UniProt No. | P03969 |
| Alternative Names | Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; FGF2; FGFB |
| Source / Host | E.coli |
| Tag | Tag free |
| Protein Length | Full Length of Mature Protein |
| Molecular Weight | 16.5 kDa |
| Formulation | Lyophilized from a 0.2 μm filtered solution containing 10mMPB, 250mMNaCl,5%mannitol and 0.01% Tween 80 ,pH 7.4 |
| Reconstitution | We recommend briefly centrifuging the vial prior to opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile deionized water to a recommended concentration of 0.1–1.0 mg/mL. If used immediately after reconstitution, glycerol is not required. If not for immediate use, after the protein is completely dissolved, add glycerol to a final concentration of 5%–50% (default: 50%), aliquot as needed, and store at -20℃/-80℃ for long-term storage. If dilution is needed during subsequent use, dilute with the same buffer as the protein, but without stabilizers (e.g., glycerol, trehalose, etc). Troubleshooting and FAQs Protein FAQs |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Lead Time | 3-7 business days |
Description
FGF2 orchestrates cell proliferation, survival, and differentiation across multiple lineages, making it a critical tool for dissecting mitogenic signaling and tissue regeneration pathways. This tag-free bovine ortholog, spanning the complete mature sequence (aa 10–155), demonstrates robust bioactivity with an ED50 ≤2.0 ng/mL in NIH3T3 fibroblast proliferation assays, confirming potent receptor engagement suitable for dose-response studies in stem cell expansion, neuronal differentiation protocols, and angiogenesis models. Expressed in *E. coli* to yield an unglycosylated form that retains… Read more