Pep – 1 – Chariot – Cell Penetrating Peptides – CPP

Pep-1 is a synthetic cell-penetrating peptide (CPP), and has been successfully used to deliver a variety of proteins and other biopharmaceutical macromolecules into cells in a non-disruptive manner. It is a CPP with primary amphipathicity, which results from its amino acid sequence as opposed to its folding structure. The primary structure of Pep-1: Chariot comprises three main domains: a tryptophan-rich, hydrophobic domain, and a hydrophilic domain derived from an NLS (nuclear localisation signal) of SV40 (simian virus 40) large T-antigen, and a spacer.

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