E. coli 16S-FAM-Ahx Probe
E. coli 16S CENPB PNA FISH PROBES
The E. coli 16S-FAM-Ahx Probe is a sequence-specific PNA probe developed for the selective detection of Escherichia coli, including O157:H7 strains. The probe sequence targets a defined region within the bacterial 16S rRNA, enabling accurate identification. It is designed for use in PNA-FISH, a rapid technique that detects microorganisms directly by hybridizing to intracellular rRNA.
Main characteristics
This PNA probe enables sequence-specific detection of E. coli O157:H7 and is designed for use in PNA-FISH applications targeting rRNA. It provides high specificity for regions associated with enterohemorrhagic E. coli (EHEC) and benefits from the neutral PNA backbone, which ensures strong and stable hybridization. The method allows rapid and sensitive detection without the need for PCR amplification, making it well suited for fluorescence-based microbial identification workflows. The probe is conjugated with FAM (Ex/Em = 495/520 nm), allowing direct detection under fluorescence microscopy. An Ahx spacer (6-aminohexanoic acid, CAS: 60-32-2) is included to improve accessibility of the binding region and enhance hybridization efficiency, while a terminal glycine can be incorporated to increase flexibility and support optimal binding without affecting specificity.
Applications
- Identification of E. coli O157:H7 in clinical microbiology samples
- Screening for pathogenic E. coli in food safety testing
- Monitoring of environmental samples such as water and agricultural sources
- Use in PNA-FISH methods for microbial detection
- Research on enterohemorrhagic E. coli and similar pathogens
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Technical specification
| Sequency : | FAM-Ahx-caacacacagtgtc-G | |
| MW : | 4294.11 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB346 – 25nmol | 25 nmol | 448 | 537 |
| SB346 – 50 nmol | 50 nmol | 652 | 782 |
For more information about PNA, please visit our dedicated page.
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A biotin-labeled version of this peptide is also available as an alternative to the FAM-conjugated format, offering flexibility for different detection methods.Tap the button below to view the Biotin-conjugated product.
Custom PNA Services
If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.