Chloroplast pPNA
Plastid 16S rRNA PNA PCR blocker
Chloroplast pPNA is a PNA-based PCR blocker designed to specifically bind a conserved chloroplast 16S rRNA sequence in plants (ggctcaaccctggacag), preventing its amplification during PCR and NGS workflows. By reducing interference from chloroplast-derived sequences, it enhances the detection of nuclear genes in complex plant DNA and RNA samples. Thanks to the intrinsic properties of PNA, including high thermal stability, strong sequence specificity, and resistance to enzymatic degradation, pPNA-S is well suited for plant metagenomics, PCR multiplexing, and transcriptomic analyses, especially in samples rich in organelle RNA.
Main characteristics
Chloroplast pPNA PCR blocker specifically targets conserved regions of chloroplast 16S rRNA in plants, helping to limit unwanted amplification during PCR. By reducing chloroplast-derived interference, it improves the amplification and detection of nuclear genes. Its PNA backbone provides strong and selective binding, along with high resistance to enzymatic degradation. It is well suited for plant research and metagenomic applications, and can be easily integrated into both standard and high-throughput PCR workflows. A terminal glycine is added to the PNA structure to improve flexibility and reduce steric hindrance, which can help optimize binding efficiency without affecting specificity.
Applications
- Reduces chloroplast rRNA amplification in plant PCR workflows
- Improves nuclear gene profiling in chloroplast-rich tissues
- Supports RNA-seq and metagenomic enrichment
- Enables multi-target PCR in plant genetics and botany
- Applicable to agricultural genomics and biodiversity studies
- Enhances NGS workflows by depleting organelle DNA
Technical specification
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Sequency : | ggctcaaccctggacag-G |
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MW : | 4672.42 g/mol |
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Purity : | > 95% |
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Counter-Ion : | TFA Salts |
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Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB337 – 25nmol | 25 nmol | 399 | 479 |
| SB337 – 50 nmol | 50 nmol | 644 | 772 |
For more information about PNA, please visit our dedicated page.
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Custom PNA Services
If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.