CENPB FAM-Ahx Probe
CENPB Pan-Centromere PNA FISH Probe
The CENPB FAM-Ahx probe is designed to recognize the CENP-B box, a conserved motif located within alpha-satellite DNA at human centromeres. This region plays a key role in centromere function, as it is involved in the binding of the CENP-B protein, which contributes to centromere structure and stability. The probe binds specifically to centromeric regions across human and mouse chromosomes, with the exception of the Y chromosome. The reverse complementary sequence (5′ to 3′) is tcccgtttccaacgaat.
Main characteristics
The CENPB probe is commonly used in Fluorescence In Situ Hybridization (FISH) to detect and visualize centromeric regions by specifically recognizing the CENP-B box. This allows researchers to monitor centromere behavior during cell division and study chromosome segregation and structural integrity. Since CENP-B is essential for the organization of centromeric chromatin and proper kinetochore assembly, the probe is a valuable tool for analyzing chromosome dynamics during mitosis. The probe is conjugated with FAM (Ex/Em = 495/520 nm), allowing direct detection under fluorescence microscopy. An Ahx spacer (6-aminohexanoic acid, CAS: 60-32-2) is introduced to separate the fluorophore from the recognition sequence, improving accessibility and hybridization efficiency. Additionally, a terminal glycine may be incorporated into the PNA structure to increase molecular flexibility and support optimal binding performance without compromising specificity.
Applications
- Genome-wide identification of centromeric regions
- Detection of chromosomal abnormalities in oncology
- Screening for aneuploidies before and after birth
- Assessment of genomic stability in stem cell studies
- Analysis of chromosomal variations using hybridization techniques
- Detailed chromosome structure analysis at high resolution
Technical specification
| Sequency : | FAM-Ahx-attcgttggaaacggga-G | |
| MW : | 5237.95 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB343 – 25nmol | 25 nmol | 499 | 599 |
| SB343 – 50 nmol | 50 nmol | 744 | 892 |
For more information about PNA, please visit our dedicated page.
Explore our PNA Catalog products. Click HERE to discover additional reference compounds.
Custom PNA Services
If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.