(CAG)5-FAM-Ahx
CAG Repeat PNA Probe
(CAG)5-FAM-Ahx probe is a synthetic PNA probe designed to recognize CAG trinucleotide repeat sequences and is labeled with FAM, enabling green fluorescence detection in FISH experiments. These repeats are associated with disorders such as Myotonic Dystrophy Type 1 (DM1) and Huntington’s disease, making the probe useful in both research and diagnostic applications. Its PNA backbone ensures high binding specificity and strong resistance to enzymatic degradation. The FAM fluorophore provides a bright and clear signal in the green spectrum, allowing accurate visualization and high-resolution mapping of repeat regions.
Main characteristics
(CAG)5-FAM-Ahx probe specifically recognizes (CAG)n repeat sequences associated with repeat expansion disorders and is labeled with FAM (Ex/Em = 495/520 nm), enabling clear green fluorescence detection under microscopy. Its PNA backbone ensures strong binding specificity, high stability, and minimal background signal. An Ahx spacer (6-aminohexanoic acid, CAS: 60-32-2) is included to improve accessibility of the binding region, while a terminal glycine can be added to enhance molecular flexibility and support efficient hybridization. The probe performs reliably in both metaphase and interphase cells and is suitable for single-probe or multiplex FISH applications.
Applications
- Detection of CAG repeat expansions using FISH techniques
- Support for studies and diagnosis of DM1 and Huntington’s disease
- Cytogenetic analysis of trinucleotide repeat–related disorders
- Visualization and mapping of repeat regions on chromosomes
- Co-localization studies with additional genetic or repeat markers
- Research on genome instability and neurodegenerative diseases
Technical specification
| Sequency : | FAM-Ahx-cagcagcagcagcag-G | |
| MW : | 4214.62 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB345 – 25nmol | 25 nmol | 465 | 558 |
| SB345 – 50 nmol | 50 nmol | 682 | 819 |
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If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.