NLS-StAx-h
Wnt Signaling & Transcriptional Regulation Peptide
NLS-StAx-h is a cell-permeable hydrocarbon-stapled α-helical peptide designed to inhibit protein protein interactions (PPI) involving β-catenin at the terminal stage of the Wnt signaling pathway. NLS-StAx-h is an optimized variant of the original StAx stapled peptide, incorporating homo-arginine substitutions and an N-terminal nuclear localization sequence (NLS) to enhance cellular and nuclear uptake. This peptide is widely used as a research tool to study Wnt-dependent transcription and β-catenin mediated signaling.
Disease Target
Targets Wnt-driven cancers including colorectal cancer (≈90% Wnt-mutated), melanoma, and leukemias by blocking nuclear β-catenin–mediated oncogenic transcription.
Stapling Strategy
NLS-StAx-h is synthesized using Fmoc-(S)-2-(4-pentenyl)alanine (Fmoc-(S5)-OH, CAS: 288617-73-2), and incorporated at i,i+4 positions (13,17) during solid-phase peptide synthesis (SPPS). A ruthenium-catalyzed ring-closing olefin metathesis generates the (CH?)? hydrocarbon staple, stabilizing the α-helical structure and enhancing cellular uptake and target engagement.
Technical specification
| Sequency : | PKKKRKVRRWPR(S5)ILD(S5)HVRRVWR | |
| MW : | 3216.93 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized | |
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CAS number : | 2872559-21-0 |
Price
| Product | Size | Price € |
Price $ |
| SB325-1mg | 1 mg | 792 | 951 |
| SB325-5mg | 5 mg | 964 | 1157 |
| SB325-10mg | 10 mg | 1291 | 1550 |
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Custom Stapled Peptide Services
If your target is not listed, we offer custom stapled peptide design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.