Mitochondrial mPNA
Plant Mitochondria 16S rRNA Blocker
Mitochondrial mPNA is a PNA-based PCR blocker designed to bind a conserved region of plant mitochondrial 16S rRNA (sequence: ggcaagtgttcttcgga), preventing its amplification during PCR and NGS workflows. By reducing interference from mitochondrial sequences, it enables more accurate detection of nuclear genes in plant genomic studies. Its PNA backbone provides strong binding affinity, high thermal stability, and resistance to nuclease degradation, making it a reliable tool for PCR optimization, metagenomic analysis, and plant transcriptome enrichment.
Main characteristics
Mitochondrial mPNA targets conserved regions of mitochondrial 16S rRNA in plants, allowing selective suppression of unwanted amplification. Its PNA-based design ensures high specificity, strong binding affinity, and excellent stability. By minimizing off-target mitochondrial signals, it improves the detection of low-copy nuclear genes. It is compatible with standard PCR, qPCR, and amplicon-based NGS workflows, and works efficiently across a wide range of plant DNA extraction methods. A terminal glycine is added to the PNA structure to improve flexibility and reduce steric hindrance, which can help optimize binding efficiency without affecting specificity.
Applications
- Blocks mitochondrial rRNA amplification in plant PCR workflows
- Improves targeting and sequencing of nuclear DNA
- Supports plant transcriptome enrichment
- Enhances amplicon sequencing from plant tissues
- Enables organelle-free (chloroplast and mitochondrial) profiling
- Applicable to environmental and agricultural genomics
Technical specification
| Sequency : | ggcaagtgttcttcgga-G | |
| MW : | 4733.46 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB336 – 25nmol | 25 nmol | 399 | 479 |
| SB336 – 50 nmol | 50 nmol | 644 | 772 |
For more information about PNA, please visit our dedicated page.
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Custom PNA Services
If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.