Asteraceae pPNA
Asteraceae Chloroplast 16S rRNA Blocker
The Asteraceae pPNA is a specialized blocking oligonucleotide designed to suppress the amplification of chloroplast 16S rRNA in Asteraceae plant species. By targeting the conserved ggctcaaccctggacag sequence, this PNA probe significantly reduces host background noise. This enables researchers to bypass abundant chloroplast interference and achieve high-sensitivity detection of rare nuclear targets or microbial DNA.
Main characteristics
Asteraceae pPNA is designed for the targeted suppression of 16S rRNA sequences specifically within the Asteraceae family, allowing a more focused and accurate analysis. Its PNA backbone provides high thermal stability and stronger binding affinity compared to standard DNA, ensuring efficient and reliable blocking. By reducing the amplification of abundant organellar sequences, it improves the detection of low-copy nuclear genes, especially in tissues rich in chloroplast DNA. It is fully compatible with common molecular workflows, including qPCR, RT-PCR, and NGS library preparation, making it easy to integrate into existing protocols. Overall, it enhances sequencing depth and data quality by limiting non-target DNA interference. A terminal glycine can be included in the PNA structure to improve flexibility and reduce steric hindrance, helping to optimize binding without affecting specificity.
Applications
- Reduces chloroplast rRNA amplification in Asteraceae samples for clearer nuclear DNA analysis
- Improves genomic profiling in complex plant tissues by limiting organellar interference
- Supports agricultural and botanical research, including herbicide resistance studies
- Enhances the quality of amplicon sequencing and NGS data
- Minimizes multi-organelle interference in advanced sequencing workflows
Technical specification
| Sequency : | ggctcaactctggacag-G | |
| MW : | 4953.69 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB327 – 25nmol | 25 nmol | 399 | 479 |
| SB327 – 50 nmol | 50 nmol | 644 | 772 |
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Custom PNA Services
If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.