Alpha-Satellite Centromere PNA Probe
Centromere detection by FISH
Alpha-Satellite Centromere PNA Probe is designed to recognize and bind centromeric regions across human chromosomes. Centromeres play a key role in ensuring correct separation of sister chromatids during cell division. This probe specifically targets alpha-satellite DNA, a repetitive sequence characteristic of centromeres, allowing accurate identification of these regions in different experimental applications. The reverse complementary sequence (5′ to 3′) is aatgcttctgtctagttt.
Main characteristics
Alpha-Satellite Centromere PNA Probe is widely applied in Fluorescence In Situ Hybridization (FISH) to detect and visualize centromeric regions. Thanks to its uncharged PNA backbone, it shows stronger and more stable binding to DNA compared to traditional DNA probes, ensuring high specificity. Its ability to recognize centromeres across all human chromosomes makes it a useful tool for a broad range of cytogenetic analyses. The probe is conjugated with FAM (Ex/Em = 495/520 nm), allowing direct detection under fluorescence microscopy. An Ahx spacer (6-aminohexanoic acid, CAS: 60-32-2) is introduced to separate the fluorophore from the recognition sequence, improving accessibility and hybridization efficiency. Additionally, a terminal glycine may be incorporated into the PNA structure to increase molecular flexibility and support optimal binding performance without compromising specificity.
Applications
- Detection of chromosomal abnormalities, including aneuploidies in cancer and genetic disorders
- Chromosome counting and centromere identification in cytogenetic analyses
- Evaluation of genome stability in disease-related research
- Support for karyotyping and chromosomal integrity studies
- Investigation of chromosome segregation defects during cell division
- Useful in both clinical diagnostics and basic cytogenetic research
Technical specification
| Sequency : | FAM-Ahx-aaactagacagaagcatt-G | |
| MW : | 5450.21 g/mol | |
| Purity : | > 95% | |
| Counter-Ion : | TFA Salts | |
| Delivery format : | Lyophilized |
Price
| Product | Size | Price € |
Price $ |
| SB344 – 25nmol | 25 nmol | 516 | 619 |
| SB344 – 50 nmol | 50 nmol | 774 | 929 |
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Custom PNA Services
If your target is not listed, we offer custom PNA design and synthesis. Whether you require sequence optimization, specific modifications, or larger production quantities, our team can support your project. Submit your project details and we will provide a personalized proposal.